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In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone | Animal Sciences

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In-vitro and in-vivo biological activity of recombinant yellowtail kingfish (Seriola lalandi) follicle stimulating hormone

Citation:

Sanchís-Benlloch, P. J. ; Nocillado, J. ; Ladisa, C. ; Aizen, J. ; Miller, A. ; Shpilman, M. ; Levavi-Sivan, B. ; Ventura, T. ; Elizur, A. . In-Vitro And In-Vivo Biological Activity Of Recombinant Yellowtail Kingfish (Seriola Lalandi) Follicle Stimulating Hormone. General and Comparative Endocrinology 2017, 241, 41 - 49.

Abstract:

Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.

Notes:

International Symposium on Reproductive Biology and Comparative Endocrinology

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