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Department of Animal Sciences
The Robert H. Smith Faculty
of Agricultural, Food & Environment

The Hebrew University of Jerusalem.

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Phone: +972-(0)8-9489119;
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Novel non-methylated furan fatty acids in fish from a zero discharge aquaculture system


Vetter, W. ; Ulms, K. ; Wendlinger, C. ; van Rijn, J. Novel non-methylated furan fatty acids in fish from a zero discharge aquaculture system. 2016, 2 8 - 14.

Date Published:



BackgroundFuran fatty acids (F-acids) are valuable minor fatty acids which are appraised for their protective role against lipid oxidation of polyunsaturated fatty acids (PUFAs). The most relevant dietary source for F-acids is fish with the predominant occurrence of up to five dimethyl- or monomethyl-substituted homologues. During the screening of fish from a zero discharge aquaculture (ZDA) system we noted the potential presence of unusual F-acids. Methods We developed a method by gas chromatography with mass spectrometry operated in the selected ion monitoring mode for elucidation of the structures of the uncommon F-acids. Results Carp from the ZDA system contained seven non-methylated F-acids with dominance of 8-(5-hexylfuran-2-yl)-octanoic acid (8F6). Non-methylated F-acids have never been detected before in fish. Subsequent analysis of other fish species and a batch of the fish feed confirmed the presence of non-methylated F-acids. Conclusions F-acids in fish are derived from the feed. Our investigation indicates that more emphasis should be put on the F-acid concentrations in fish from aquaculture, which appears to depend on the quality of the fish feed.